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. 2018 Jun 11;9:1327. doi: 10.3389/fimmu.2018.01327

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Copyright © 2018 Ma, Li, Fan, Feng, Lin, Xiang and Shao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Trafficking analysis of Danio rerio DDX41 (DrDDX41) and identification of the nuclear export signal (NES) motif within it. (A) Schematic diagram of full-length and the NES-mutated DrDDX41. (B) Trafficking of DrDDX41 in response to poly(dA:dT) stimulation at different hours post stimulation (hps) and the contribution of NES motif to the trafficking. Stimulation with poly(dA:dT) induced transportation of DrDDX41 (full-length, a) from nucleus to cytoplasm. Meanwhile, the NES-like-mutant DrDDX41 (NES-M, b) remained in the nucleus without any response to poly(dA:dT) stimulation. Scale bars represent 5 µm. Images were captured under a laser-scanning confocal microscope (Zeiss LSM-710; original magnification, 630×).