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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Kidney Int. 2017 Jul 18;92(5):1130–1144. doi: 10.1016/j.kint.2017.04.027

Figure 7. Endogenous FPC is an N-glycosylated protein.

Figure 7

(a) Total lysates from P30 Pkhd1Flox67HA and Pkhd1wt kidneys were treated with PNGaseF or EndoH as indicated. Western blots were probed with anti-HA antibody (upper panel) or mFPC-ct (lower panel). Mature FPC-HA is resolved as a doublet but the predominant 500kDa band shifts down to ~450kDa upon treatment with PNGaseF. A small fraction of FPC is EndoH resistant (black arrow, FPC-HAR). The red arrow indicates the EndoH sensitive fragment (FPC-HAS). (b) Total lysates were prepared from DBA-positive renal epithelial cells isolated from Pkhd1Flox67HA and Pkhd1wt kidneys. Lysates were treated with PNGaseF or EndoH and Western blots were probed with anti-HA (upper panel) or mFPC-ct (lower panel). In these cells a small fraction of FPC is EndoH resistant (black arrow, FPC-HAR).