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. Author manuscript; available in PMC: 2019 Jan 10.
Published in final edited form as: Cell Host Microbe. 2017 Dec 21;23(1):41–53.e4. doi: 10.1016/j.chom.2017.11.003

Figure 2. Enrichment of HFD with inulin increased epithelial cell proliferation and prevented gut atrophy.

Figure 2

C57BL/6 male mice were fed chow, HFD, or HFD supplemented with cellulose or inulin for 4 weeks. A) Colon length (n=10). B) Colon mass (n=10). C) The mRNA was extracted from colon of mice fed with chow (n=4), HFD supplemeted with cellulose (n=8) or inulin (n=8), and expression level of ZO-1, Occludin and Claudin-2 was analyzed by RT-PCR. D) Colon histopathologic appearance by H&E staining. Scale bars, 200 μm. E) Colon crypt length (n=5), as shown by a double- headed arrow, was measured. F–G) BrdU was injected intraperitoneally 24 h before euthanizing (n=5). Visualization of BrdU positive cells in proximal colon by fluorescence microscopy following staining with FITC-anti-BrdU (F). Scale bars, 100 μm. Enumeration of BrdU positive cells (G). H–I) The paneth cells in ileum (n=5) were stained for lysozyme (H), and the number of paneth cells per crypt was counted (I), scale bars, 50 μm. Data were expressed as mean ± SEM. Statistical significance was assessed by unpaired Student t test. *p<0.05; **p<0.01. See also Figure S2.