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. Author manuscript; available in PMC: 2019 Jan 10.
Published in final edited form as: Cell Host Microbe. 2017 Dec 21;23(1):41–53.e4. doi: 10.1016/j.chom.2017.11.003

Figure 3. Inulin restored microbiota loads and prevented HFD-induced microbiota encroachment.

Figure 3

C57BL/6 male mice were fed chow, HFD, or HFD supplemented with cellulose or inulin for 4 weeks and feces collected at 28 days post diets treatment. A) Levels of fecal bacterial DNA were quantitated by qPCR (n=5). B–E) Fecal microbiota composition was analyzed by 16S RNA sequencing (n=4–5). B–D displays relative abundance of bacteria at phylum level. E Global composition as expressed by UniFrac PCoA analysis. F–G) Analysis of microbiota-mucus-epithelial localization in transverse colon via carnoy fixation, FISH, immunohistochemical staining (F). Scale bars, 20 μm. Bacterial epithelial distance was quantified (G). H) Quantitation of colonic Reg3γ by RT-PCR. Data were expressed as mean ± SEM. Statistical significance was assessed by unpaired Student t test. *p<0.05; **p<0.01. See also Figure S3.