Antibodies |
Anti-Ki67 |
Abcam |
Cat.# Ab15580 |
Anti-PCNA |
Cell Signaling Technology |
Cat.# 2586S |
Anti-Lysozyme |
Abcam |
Cat.# ab108508 |
FITC labeled anti-BrdU |
Abcam |
Cat.# Ab74545 |
Anti-Mucin 2 |
Santa Cruz Biotechnology |
Cat.# H-300 |
Anti-GLP1 |
Abcam |
Cat.# Ab22625 |
Alexa Fluor 488 Goat Anti-Rabbit IgG |
Invitrogen |
Cat.# A11008 |
Chemicals, Peptides, and Recombinant Proteins |
D-(+)-GLUCOSE |
Sigma-Aldrich |
Cat.# G8270-1KG |
Insulin |
Sigma-Aldrich |
Cat.# 12643-25MG |
Oil red O |
Sigma-Aldrich |
Cat.# O0625-100G |
Sodium acetate |
Sigma-Aldrich |
Cat.# S2889-250G |
Sodium butyrate |
Sigma-Aldrich |
Cat.# 303410-100G |
Sodium propionate |
Sigma-Aldrich |
Cat.# P1880-500G |
Ampicillin |
Sigma-Aldrich |
Cat.# A9518-100G |
Vancomycin |
Sigma-Aldrich |
Cat.# V2002-250MG |
Neomycin |
Sigma-Aldrich |
Cat.# N1876-100G |
Metronidazole |
Sigma-Aldrich |
Cat.# M1547-25G |
Critical Commercial Assays |
Free Fatty Acid Assay Kit |
Abcam |
Cat.# Ab65341 |
Triglycerides Reagent |
Thermo Scientific |
Lot. # 723341 |
Cholesterol Reagent |
Thermo Scientific |
Lot. # 848958 |
IL-22 ELISA Kit |
R&D Systems |
Cat.# DY582 |
One-Step RT–PCR Kit with SYBR Green |
Bio-Rad |
Cat. # 172-5151 |
QIAamp DNA Stool Mini Kit |
Qiagen |
Cat.# 51504 |
QuantiFast SYBR Green PCR kit |
Bio-Rad |
Cat.# 204054 |
Experimental Models: Organisms/Strains |
Mice: C57BL/6 |
The Jackson Laboratory |
Cat. # 000664 |
Mice: Rag1 knockout |
The Jackson Laboratory |
Cat. # 002216 |
Mice: GPR43 knockout |
The Jackson Laboratory |
Cat. # 027241 |
Mice: Rag2−/−IL2rg−/−
|
The Jackson Laboratory |
Cat. # 014593 |
Mice: IL-22 knockout |
Genentech, Inc |
NA |
Mice: Swiss-Webster mice |
Charles River Laboratories |
Cat. # 024 |
Oligonucleotides |
See Figure S2
|
This study |
|
Software and Algorithms |
GraphPad Prism |
GraphPad Software |
NA |
Image J |
NIH |
NA |
Other |
halloidin–Tetramethylrhodamine B isothiocyanate |
Sigma-Aldrich |
Cat.# P1951-.1MG |
β-acid |
S.S. Steiner, Inc |
NA |
DAPI Fluoromount-G® Mounting Medium |
SouthernBiotech |
Cat.# 0100-20 |
Rodent chow |
LabDiet |
Cat. # 5001 |
Standard high-fat diet |
Research Diets, Inc. |
Cat. # D12492 |
Inulin-enriched high-fat diet |
Research Diets, Inc. |
Cat. # D13081106 |
Cellulose-enriched high-fat diet |
Research Diets, Inc. |
Cat. # D13081107 |