Fig. 3. Schematic display of the set of rationally designed supramolecular hGQ DNAzyme dopamine binding aptamer (DBA) nucleoapzyme systems that were implemented in this study. Panel I: schematic depiction of the supramolecular nucleoapzyme structures that contain no Pi unit (system 0), or flexible units between the double-strand L/L′ region and the hGQ and DBA units (systems 4r, 5r, and 6r). The inability of the hGQ-strands to form a triplex with the stem of hairpin A is shown by the blue bars. Panel II: schematic depiction of nucleoapzyme structures in which the hGQ strand is fixed on the stem-region of hairpin A (3t, 4t, 5t, and 6t). In each structure, the green bars show the DNA-triplex section between the hGQ strand and the stem of hairpin A. Panel III: control systems that were used in this study, i.e. supramolecular architectures that contain bases in addition to the triplex-section (7t, 8t, and 9t), and the single-strand extended hGQ structure ss9. In all DBA structures, the nucleotides that form the binding site are indicated; the green oval in the DBA highlights the 5′-end facing opening of the binding pocket.
