Figure 3.

Axitinib and AB1010 induced apoptosis in h-5637 cells. (A) h-5637 and (B) K9TCC#1Lillie cells were treated with 5 μM axitinib and 5 μM AB1010 for 24 hours and cells were stained with Annexin V-FITC and PI followed by flow cytometry analysis. Percentage of apoptotic cells (positive for both Annexin V-FITC and PI staining) of treatment groups were normalized to the control (DMSO). Both axitinib and AB1010 significantly increased apoptosis in h-5637 and K9TCC#1Lillie cells. Values represent mean ± SE of two replicates from three independent experiments; paired Student’s t-test, *p<0.05, and **p<0.01.

Abbreviations: FITC, fluorescein isothiocyanate; PI, propidium iodide; DMSO, dimethyl sulfoxide; Ctrl, control.