Figure 7.

Co-treatment of AB with Indo inhibited PGE₂ levels produced by bladder TCC cells. (A) h-5637 and (B) K9TCC#1Lillie cells were treated with 5 μM AB and 50 μM Indo alone or in combination (AB + Indo). PGE₂ levels in cell culture media collected 24 hours after treatment were determined by ELISA. Indo significantly reduced the production of PGE₂ by bladder TCC cells. Co-treatment of AB with Indo significantly decreased the AB-induced PGE₂ levels produced by K9TCC#1Lillie cells. Values represent mean ± SE of three replicates of three independent experiments; paired Student’s t-test was used to compare treatments to the control groups, *p<0.05 and ***p<0.001.

Abbreviations: AB, AB1010; Indo, indomethacin; TCC, transitional cell carcinoma; ELISA, enzyme-linked immunosorbent assay; PGE₂, prostaglandin E2.