A, H&E-stained sections of KPC cell invasion into fibroblast-conditioned collagen matrix after 8 days, which included treatment with AT13148, Y27632 or GM6001 as indicated. The invasion index was normalized to DMSO control conditions, with means ± SEM shown from n = 3 independent experiments. Statistical significance was determined using one-way ANOVA and post-hoc Dunnett’s multiple comparison test. *, p<0.05; **, p<0.01; ***, p<0.001. B, H&E-stained sections of TKCC5 cell invasion into fibroblast-conditioned collagen matrix after 8 days, which included treatment with AT13148. The invasion index was normalized to DMSO control conditions, with means ± SEM shown from n = 3 independent experiments. Statistical significance was determined using unpaired Student’s t-test. ***, p<0.001.