(A to C) Four representative blockface images (left to right) with integration of MALDI IMS signal for the S100A8 subunit of calprotectin (CP) (m/z = 10,164, blue) and LA-ICP-MS signal (orange-yellow) for calcium (Ca; A), manganese (Mn; B), and zinc (Zn; C). Insets: Enlarged images of the kidney and immediate surrounding tissue. (D) The MALDI IMS imaging volume for calprotectin encompassing the infected right kidney was co-registered to the LA-ICP-MS imaging volume for Ca, Mn, or Zn, displayed obliquely to delineate calprotectin and element distribution throughout the kidney. Heat maps depict minimum and maximum values in arbitrary units. See also movie S9.