1,25D therapy restores expression of markers of perilacunar and canalicular remodeling in Hyp mice. mRNA and protein expression of markers of osteocyte lacunocanalicular remodeling was evaluated in d30 osteocyte-enriched femoral cortices. (A) FGF23, MEPE, and DMP1 mRNA expression. (B) mRNA expression of genes important for bone resorption and canalicular remodeling. (C) Immunohistochemical analysis of cathepsin K in d30 tibia and calvaria. (D) Immunohistochemistry for MMP13 in d30 tibia and calvaria. Data are representative of that obtained from 5 mice per genotype/treatment group. *p < 0.05, #p < 0.05 versus Hyp, ap < 0.05 versus Daily 1,25D.