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. 2018 May 1;293(24):9188–9197. doi: 10.1074/jbc.RA118.002851

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© 2018 Zuo et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 1. — SETD2 expression and H3K36me3 distribution during spermatogenesis. A, immunofluorescence staining for SETD2 (green), rhodamine-labeled PNA (a marker for the acrosome; red), and DAPI (blue) in sections of adult mouse testes. SETD2 immunoreactivity was mostly seen in the nucleus of pachytene spermatocytes and round spermatids. B, immunofluorescent detection for H3K36me3 (green), rhodamine-labeled PNA (red), and DAPI (blue) in sections of adult mouse testes. H3K36me3 staining was detected in the nucleus of all stage of spermatocytes and round as well as elongating spermatids (scale bar, 40 μm).