Figure 4.

A network of interactions is formed between the USR, N-clamp, and core YTH domain. A, co-crystal structure of Mmi1 USR–YTH (residues 315–488 modeled) with DSR RNA. Cartoon representation shows protein in blue and RNA in yellow. The USR is shown in darker blue. B, conformation of the USR is stabilized by hydrophobic contacts with the core YTH domain and N-clamp. Residues labeled in turquoise either appear or show large chemical shift changes on RNA binding in NMR experiments (see Fig. 5).