Table 1.
Comparison of calculated binding parameters for Mmi1-RNA interactions
| Construct (residues) | Fluorescence polarization, calculated KD (nm) |
SwitchSENSE |
|||
|---|---|---|---|---|---|
| (50 mm NaCl) | (150 mm NaCl) | Calculated KD (nm) (40 mm NaCI) | kon (× 105 m−1 s−1) | koff (×10−3 s−1) | |
| YTH (327–488) | 42 ± 18 | 154 ± 85 | 16 ± 2 | 3.0 ± 0.4 | 4.90 ± 0.04 |
| USR-YTH (282–488) | 0.14 ± 0.01 | 6.1 ± 0.7 | 0.090 ± 0.006 | 8.3 ± 0.5 | 0.075 ± 0.0005 |
| YTH-ΔC (327–483) | 160 ± 80 | 12 ± 5 | 190 ± 20 | ||
| YTH-ΔN (347–488) | 15,000 ± 3,000 | 0.81 ± 0.13 | 1200 ± 200 | ||
| YTH-ΔNΔC (347–483) | 51,000 ± 34,000 | 0.38 ± 0.25 | 1900 ± 700 | ||