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Establishment of a spontaneously immortalized MSC line. A, FACS analysis of p58 MSC for surface markers of MSC (CD105, CD44, and Sca1) and hematopoietic cells (CD45 and CD11b). Autofluorescence is shown in red (n = 3). B, bright field microscopy of MSC. C, differentiation of p58 MSC to adipocytes and osteoblasts stained with Oil Red O (adipocytes, fat vesicles shown in red) and Alizarin Red S (osteoblasts, orange-red) (n = 4).
