Figure 4.

Rho GTPases regulate TGFβ-induced expression of αSMA but not of col1a1. A, D, and E, qRT-PCR analysis for indicated genes of RhoA-KO, Rac1-KO, Cdc42-KO, and WT cells after treatment for 24 h (A and D) or 3 days (E) with TGFβ (black bars, 24-h TGFβ; gray bars, untreated; n = 6/6). B, C, and F–H, representative Western blotting and quantification of lysates of indicated cells for αSMA (B and C) and collagen I (F–H) (black bars, 24-h TGFβ; gray bars, untreated; n = 4/4). ****, p < 0.0001.