Figure 6.

Cofilin is not required for TGFβ-induced expression of αSMA in MSC. A, B, D, E, G–K, and M–O, qRT-PCR analysis for indicated genes of WT cells treated with latrunculin A (latA) or jasplakinolide (jasplak) (A and B), Arpc2-KO (D and E), cofilin-KO (G and H), Limk1-KO (I and M), Limk2-KO (J and M), Limk1/2 KO (K and M), and MRTFa KO (N and O) (n = 4/4). C, representative Western blotting for the indicated proteins of MSC with knockout of Arpc2 or cofilin. F, upper, bright field microscopy of indicated MSC. Lower, F-actin (white) of indicated MSC, detected by fluorescently labeled phalloidin. Nuclear counterstaining (blue) by DAPI is shown. L, Western blotting for pCofilin of MSC with indicated KO. (A–O, black bars, 24-h TGFβ; gray bars, untreated). ****, p < 0.0001.