Figure 1.
Vpr interacts with Cep78 and EDD-DYRK2-DDB1DCAF1 through DCAF1. A, HEK293 cells were transfected with nonspecific (NS) or DCAF1 siRNA followed by plasmid expressing HA-Vpr(WT). Lysates were immunoprecipitated (IP) with an anti-HA antibody and Western-blotted with the indicated antibodies. IN, input. α-Tubulin was used as loading control. B, HEK293 cells were transfected with plasmid expressing HA, HA-Vpr(WT), or HA-Vpr mutant refractory to DCAF1 binding (Q65R). Lysates were immunoprecipitated with an anti-HA antibody and Western-blotted with the indicated antibodies. C, HEK293 cells were co-transfected with plasmids expressing FLAG-Cep78 and HA or HA-Vpr(WT). Lysates were immunoprecipitated with an anti-FLAG antibody and Western-blotted with the indicated antibodies.