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. 2018 May 3;293(24):9448–9460. doi: 10.1074/jbc.RA117.001444

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Figure 8. — Degradation of CP110 induced by Vpr or Vpr(R80A) can be overcome by Cep78 expression. A, HEK293 cells were transfected with plasmids expressing HA and FLAG, HA-Vpr(WT) and FLAG, or HA-Vpr(WT) and FLAG-Cep78. Lysates were Western-blotted with the indicated antibodies. α-Tubulin was used as loading control. B, normalized CP110 protein level. The mean (thick open line) and standard error (bar) of three independent experiments (○) are shown in the graph. *, p < 0.01. C, HEK293 cells were transfected with plasmids expressing HA and FLAG, HA-Vpr(R80A) and FLAG, or HA-Vpr(R80A) and FLAG-Cep78. Lysates were Western-blotted with the indicated antibodies. α-Tubulin was used as loading control. D, normalized CP110 protein level. The mean (thick open line) and standard error (bar) of three independent experiments (○) are shown in the graph. *, p < 0.01.