Figure 1.

Effect of uPA on the synaptic expression of ezrin. A–F, representative immunoblots (A, C, and E) and mean intensity of the band (B, D, and F) of ezrin (A and E) and radixin (C) expression in synaptoneurosomes prepared from WT cerebral cortical neurons incubated 0–60 min with 5 nm of uPA (A–F), or its N-terminal fragment (ATF; E and F). The data are expressed as means ± S.E. for n = 4 observations. G and H, representative Western blotting analysis (G) and mean intensity of the band (H) of ezrin expression in whole-cell extracts prepared from WT cerebral cortical neurons incubated 0–60 min with 5 nm of uPA. The data are expressed as means ± S.E. for n = 4 observations. I and J, representative Western blotting analysis and mean intensity of the band of ezrin expression in synaptoneurosomes prepared from WT cerebral cortical neurons incubated 60 min with 5 nm of uPA, alone or in the presence of 10 μg/ml of puromycin. The data are expressed as means ± S.E. for n = 4 observations.