Figure 1.
Generation of PTENK13R, D384V mice. A, mutations were introduced into the PTEN gene to substitute lysine 13 (K13) to arginine (R) in the N-terminal PIP2-binding domain (PB) and aspartate 384 (D384) to valine (V) in the C-terminal tail. B, DNA sequencing confirmed the K13R mutation (AAA changed to AGA) and D384V mutation (GAC to GTG). C, general appearance and body weight of PTENK13R, D384V and littermate control mice at 8–10 weeks old. Bars represent the mean ± S.E. (n = 8 male mice). Statistical analysis was performed using the Student's t test. Scale bar in C, 1 cm.