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Chondrodysplasia in the limbs of Smad4 mutant mice. A–F, Alcian blue staining on the control (A, C, and E) and mutant forelimbs (B, D, and F) at E12.5–14.5. Arrows in A–F indicate humerus. G–L, RNA in situ hybridization for Lect1 and Col2a1 on the forelimb sections at E12.5 and E13.5. Arrows indicate RNA expression in humerus. M–O, Lect1, Col2a1, and Acan expression level was evaluated by qRT-PCR in the forelimbs at E12.5 and E13.5. n = 3, *, p < 0.05.
