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. 2018 Mar 19;293(24):9530–9541. doi: 10.1074/jbc.RA118.002449

Figure 2.

Figure 2.

Expression of p62 and OC marker proteins during OCG is shown by IB analyses of RIPA cell lysates from BMM-derived cells of p62+/+ (A) and p62−/− (B) origin (C57BL/6N). BMMs were stimulated with M-CSF/sRANKL for the indicated time (days (d) 0–5). M-CSF or M-CSF/sRANKL/IL-4 stimulation served as macrophage and negative controls, respectively. Lysates were subjected to IB analysis with Abs against p62, Nfatc1, Ctsk, TRAF6, P-NF-κB p65, NF-κB p65, and β-actin. Induction of Nfatc1 and Ctsk during OCG is independent of p62 expression. Shown IBs are representatives of replicates with comparable results.