Fig 4. Quantification of 5S-KSS-14L RNA expression by semi-quantitative RT-PCR.

On the left, PAGE analysis of the RT-PCR reactions performed on the total cellular RNA or known amounts of purified T7-transcripts, as indicated above the panel. On the right, an example of the calibration curve used for quantification of 5S-KSS-14L RNA (in red) in cybrid cell line.