Fig 5. HBV/HDV infection via human and monkey NTCP/Ntcps.

HepG2 cells were transiently transfected with human/monkey NTCP/Ntcps wild type or mutant constructs in 96 well plates and inoculated with 10,000 genome equivalents of pseudotyped HDV particles (HDVpsHBV/HDVpsWMHBV) or 2,000 HBV genome equivalents per cell. Cells were cultured for 10 days post infection and then immunostained against HDAg or HBcAg. Infected cells per well were manually counted by fluorescence microscopy. Cell counts are depicted as means ± SD of one representative experiment performed in triplicate.