Table 4.

Amino acid substitutions in the 8 segments of influenza strains isolated in Korea during 2011‐2015 seasons compared to the vaccine strains

Subtype	Seasons	Sample name	HA	NA	M1	M2	NP	NS1	NS2	PA	PB1	PB2	Genetic clade (HA based)
H3N2	H3N2 Vaccine	A/Victoria/361/2011	‐	‐	‐	‐	‐	‐	‐	‐	‐	‐
	2011‐2012	A/Suwon/C276/2012	Q33R, E50K, V186G, D188G, R255T, P273R, N278K, I149T	I222V, K258E, T329N	‐	V51I,	S359P, M374I, M440V	D209N, K229E	‐	E351D, R356K, M407I	D581N	V606A	3C.1
		A/Ansan/D16/2012	Q33R, V186G, N278K	I222V, K258E, T329N	‐	G16E, V51I, E97G	V197A, M374I	V22F, D209N, K229E	K39R	E351D, M407I	‐	M66T, R389K	3C.1
	2012‐2013	A/Ansan/D342/2013	Q33R, N145S, V186G, N278K	I20T, K258E, T329N	‐	‐	K470M	E26K, K78R, D209N, K229E	‐	M407I	R361K	Y55F, R194Q, L384I	3C.2
		A/Seoul/A468/2013	Q33R, N145S, V186G, N246Y, N278K, D160N	E221D, K258E, T329N, I392T	‐	‐	K357R	E26K, I182V, D209N, K229E	‐	M407I, V668I, N675K	M317T	‐	3C.2
		A/Cheongju/G856/2013	Q33R, N145S, V186G, N278K, D160N	E221D, K258E, T329N, I392T	‐	‐	‐	E26K, I182V, D209N, K229E	‐	G240V, M407I, V668I, N675K	‐	‐	3C.2
	H3N2 Vaccine	A/Switzerland/9715293/2013	Q33R, T128A, A138S, R142G, N145S, F159S, V186G, N225D, N262K, K326R	E221D, K258E, T329N, I392T	‐	‐	S217G	E26K, M124I, D209N, K229E	‐	Q256K, I308V, I554V, K605R, V669I, H713Y	‐	‐	3C.3a
	2013‐2014	A/Cheongju/G1629/2014	Q33R, T128A, A138S, R142G, N145S, F159S, V186G, N225D, N262K, K326R	E221D, K258E, T329N, D339N, I392T	‐	‐	S217G, S482N	E26K, M124I, D209N, K229E	‐	Q256K, I308V, I554V, K605R, V669I	‐	‐	3C.3a
		A/Ansan/D765/2014	Q33R, T128A, A138S, R142G, N145S, F159S, V186G, N225D, N262K, K326R, V73A	Y67F, E221D, K258E, T329N, I392T	‐	‐	S217G	E26K, M124I, D209N, K229E	‐	Q256K, I308V, I554V, K605R, V669I	R52K, L424M	V338I, S709N	3C.3a
		A/Cheongju/G1532/2014	Q33R, T128A, A138S, R142G, N145S, F159S, V186G, N225D, N262K, K326R	E221D, K258E, T329N, I392T	‐	‐	S217G	E26K, M124I, A202T, D209N, K229E	‐	Q256K, I308V, A455G, I554V, K605R, V669I	T400K	A559V, S643T	3C.3a
	2014‐2015	A/Cheongju/G2156/2015	Q33R, T128A, A138S, R142G, N145S, F159S, V186G, A212S, N225D, N262K, K326R, A201V	E221D, K258E, T329N, I392T	‐	‐	I136L, S217G	E26K, M124I, D209N, K229E	‐	Q256K, I308V, D396E, I554V, K605R, V669I	‐	R390K, M476L	3C.3a
		A/Seoul/A1284/2015	L3I, Q33R, N144S, N145S, F159Y, V186G, N225D, N278K, Q311H, D160N	E221D, S245N, S247T, K258E, T267K, T329N, I380V	‐	I39M	M481I	E26K, D209N, K229E	‐	N272S, D396E, M407I, V668I, N675K	‐	V63I, I589T	3C.2a
		A/Seoul/A1251/2015	L3I, Q33R, N144S, N145S, F159Y, K160T, V186G, N225D, N278K, Q311H, D160N	D151N, E221D, S245N, S247T, K258E, T267K, T329N, I380V, W383C	‐	I39M	M481I	E26K, D209N, K229E	‐	N272S, D396E, M407I, V668I, N675K	W580C	V63I, I589T	3C.2a
H1N1	H1N1 Vaccine	A/California/07/2009	‐	‐	‐	‐	‐	‐	‐	‐	‐	‐
	2012‐2013	A/Cheongju/G792/2013	P83S, S84G, S143G, K163I, G170R, S185T, A197T, S203T, A261T, G262E, H273N, I321V, E47K, S124N, I183V, V193A	L40V, N44S, S52N, N200S, V241I, N248D, T289I, Y351F, N369K	V80I, M192V, K230R	D21G	V100I, L108I	L90I, I111T, V117M, I123V, N205S	T48A	I30T, Y161F, P224S, E252V, N321K, A343T, V407I, R673S	G154D, I397M, I435T, H456Y	D195N, R293K, V344M, I354L, V731I	7

HA2 domain is indicated in bold‐italic font