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Western blot for VCAp23 (product of EBV BLRF2) in ΔBALF2 HEK293 cells, indicating that in absence of DNA replication (induced only (I)), VCAp23 is still expressed. When DNA replication is restored by trans-complementation with BALF2 (I + t), VCAp23 levels further increase. VCAp18 (product of true late BFRF3 gene), EA-D (product of early BMRF1 gene) and Tubulin blots are shown as controls.
