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. Author manuscript; available in PMC: 2018 Jun 18.
Published in final edited form as: Nature. 2012 Oct 7;491(7426):765–768. doi: 10.1038/nature11463

Figure 4. Human ESC-derived cells expanded on mesenchyme give rise to insulin-expressing, glucose-responsive cells in vivo.

Figure 4

a, Schematic depicting implantation of human ESC-derived progenitors. b, Immunofluorescence staining of human ESC-derived endoderm, passaged seven times on mesenchyme and engrafted for 3 months (top panel) or further differentiated to Pdx1+ stage and then engrafted for 2 months (bottom panel). c, Glucose-tolerance test of animals implanted with PBS or mesenchyme only, human islets or Pdx1+ pancreatic progenitors derived from unpassaged (P0), or passaged (P4 or P7) human endoderm. d, Fasting- and glucose-induced (45 min glucose) plasma human C-peptide levels. Pairs of bars represent two time points per animal; data represent mean of two technical replicates ± s.d.