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Co-expression and purification of VPS33B and GST-VIPAR. (A) Size exclusion chromatogram (Superdex 200 10/300 GL) showing purification of the VPS33B/GST-VIPAR complex from E. coli following GSH affinity purification. Fractions from the indicated peak were pooled. (B) Pooled and concentrated fractions were analyzed by SDS-PAGE. The indicated bands were confirmed by mass fingerprinting as indicated.
