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. 2018 Jun 12;12:49. doi: 10.3389/fnana.2018.00049

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Copyright © 2018 West.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Depiction of an isotropic surface probe shown in light gray in a tissue sample shown in blue. The estimate of the length density, $\hat{L_{V}}$ , is directly related to the number of times the linear features pass through the surface of the hemi-space ball probe (see Equation 1). The area of the probe is known from the formula for the surface of a sphere of known diameter, D. The volume of tissue sampled is known from the sample area times the thickness of the section (West, 2012, Figure 5.3, with permission).