Table 1.
Primer sequences used for real-time quantitative PCR.
| Gene | GenBank | Primer sequence(5′-3′) |
|---|---|---|
| accession no. | ||
| PR (pathogenesis-related protein) | Solyc01g106620.2 |
F: GAGGGCAGCCGTGCAA R: CACATTTTTCCACCAACACATTG |
| GLU (beta-1, 3-glucanase) | CK664757 |
F: GCGGTGTTCAGCCTGGATG R: AGCATGAGCAAGAAGTATGTTGTG |
| LOX (lipoxygenase) | U37840 |
F: GACTGGTCCAAGTTCACGATCC R: ATGTGCTGCCAATATAAATGGTTCC |
| PI (proteinase inhibitor) | K03291 | F: GAAAATCGTTAATTTATCCCACCG R: ACATACAAACTTTCCATCTTTACCA |
|
ACO (1-aminocyclopropane-1-carboxylate oxidase) |
X58273 |
F: GCCAAAGAGCCAAGATTTGA R: TTTTTAATTGAATTGGGATCTAAGC |
|
ACS (1-aminocyclopropane-1-carboxylate synthase) |
X59139 |
F: GGTCTTGCTGAAAATCAGCTTTGT R: AGTTGGCAATGGCCTTGAATG |
| ERF1 (ethylene-responsive factor 1) | AY044236 |
F: AGAGACCAAGGACCCCTCAT R: AGTAGAGACCAAGGACCCCTC |
| ERF2 (ethylene-responsive factor 2) | AY192368 |
F: AAGGGGTTAGGGTTTGGTTAGG R: CAAGCAATGTTCAAGGGAGGG |
| TIP41 | SGN-U321250 |
F: AGGCCTTGTCTTCGAAAGGA R: TCCTTTAGGACACTCCAACATGG |
| Actin | AB199316 |
F: TGGTCGGAATGGGACAGAAG R: CTCAGTCAGGAGAACAGGGT |