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. 2018 Jun 12;9:1157. doi: 10.3389/fmicb.2018.01157

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Copyright © 2018 Chen, Wei, Ye, Yun, Kang, Tang, Guo and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow diagram of CRISPR-Cas9 vector construction. (A) Pcmgpd, Pcmlsm3 and Tcmura3 were cloned from the genome of C. militaris. (B) The cassette Pcmgpd-cmcas9-gfp-myc was ligated into pCAMBIA0390 to build the p390cm-gpd-c9gm vector. (C) The cassette Pcmlsm3-blpR-Tcmura3 was ligated into p390cm-gpd-c9gm to build p390-blpR-cmcas9-gfp.