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The qPCR experiments were carried out in biological triplicate. Data are represented as the mean ± SEM (n = 9). Statistical analyses were performed using t-tests compared with wild-type “CM10” (***p < 0.001, ****p < 0.0001). (A) The expression level of sgRNA in the transformant C. militaris C9-sR (C. militaris:: blpR-cmcas9-gfp-HH-sgRNA-HDV). (B) The expression level of Cas9 in the transformant C. militaris C9 (C. militaris:: blpR-cmcas9-gfp).
