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. 2018 Jun 12;9:1157. doi: 10.3389/fmicb.2018.01157

Figure 3.

Figure 3

Detection of Cas9-expressing transformants. (A) Nucleic acid gel electrophoresis analysis of putative Cas9 transformants (Expected target band was 491 bp). (B) Western blot for Cas9 detection (S1–S5 represented putative transformants; CM10 represented the negative control; the expected Cas9 band was 186.5 kDa and is indicated by arrows).