Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 12;9:1157. doi: 10.3389/fmicb.2018.01157

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Chen, Wei, Ye, Yun, Kang, Tang, Guo and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Sequence design and alignment diagrams for editing cmura3 by CRISPR-Cas9 in vivo. (A) The localized sites and target sequences of sgRNA. (Apostrophe in the box represents the sgRNA scaffold sequence). (B) The sequence of donor ssDNA ortUra3-1. (The target sgRNA site was underlined; the inserted sequence was marked blue and listed separately). (C) The sequence and sequencing chromatogram for gUra3-1 deletion mutation. (D) The sequences and sequencing chromatograms for gUra3-1 replacement mutation. (E) The sequences and sequencing chromatograms for gUra3-1 insertion mutation and the alignments for the insertions. (F) The sequence and sequencing chromatogram for the gUra3-2 inserted mutation and alignment for the insertions.