Table 1.
Materials of strains and nucleic acid.
| Strains | Source |
|---|---|
| Escherichia coli strain DH5α | Purchase from Weidi Bio, Shanghai, China |
| Agrobacterium tumefaciens AGL-1 | Purchase from Weidi Bio, Shanghai, China |
| Cordyceps militaris CM10 | Purchase from Lucky Mushroom Garden (http://www.bjjixunyuan.com), Beijing, China |
| C. militaris C9 | This study, C. militaris:: blpR-cmcas9-gfp |
| VECTORS | |
| pMD18T | Purchase from Takara, Beijing, China |
| pCAMBIA03301 | Purchase from Cambia, Queensland, Australia |
| pCAMBIA0390 | Purchase from Cambia, Queensland, Australia |
| cmgpd + T, cmlsm3 + T, cmura3 + T | This study, Table 2, Figure 1 |
| p390cm-gpd-c9gm | This study, Table 2, Figure 1 |
| PGH-gfp, PGH-CmCas9 | This study, synthesized by GENEray, Shanghai, China |
| p390-blpR-cmcas9 | This study, Table 2, Figure 1 |
| p390-blpR-gRNA-cmcas9 | |
| DNAS | |
| Lcmlsm3-blp-Tcmura3 | This study, Table 2, Figure 1 |
| ortUra3-1 | This study, Table S4, Figure 5B |
| RNAS | |
| sgRNA-gUra3-1 | This study, Table S5, Figure 5 |
| sgRNA-gUra3-2 | This study, Table S5, Figure 5 |
| Genes | Description |
| cmcas9 | From S. pyogenes Cas-9 but codon-optimized for C. militaris |
| gfp | Green fluorescent protein eGFP |
| myc | Nuclear localization signal c-Myc (PAAKRVKLD) |
| cmgpd | Glycerol-3-phosphate dehydrogenase of C. militaris (Accession No. NW_006271970.1) |
| cmlsm3 | U6 small nuclear ribonucleoprotein of C. militaris (Accession No. NW_006271974.1) |
| cmura3 | Orotidine 5′-phosphate decarboxylase of C. militaris (Accession No. NW_006271972.1) |
| blpR | Phosphinothricin acetyltransferase |