Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 12;9:1266. doi: 10.3389/fmicb.2018.01266

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Zhang, Zhang, Fu, Liu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Estrogen-induced Ca²⁺ fluxes in T. gondii. (A) Video microscopy of intracellular parasites expressing GCaMP6f, following the addition of estradiol at 0 s. (B) Intracellular Ca²⁺ concentrations monitored over time in GCaMP6f-RH parasites (extracellular tachyzoites) stimulated with 10^-8 M estradiol. (C) Intracellular Ca²⁺ fluxes of GCaMP6f-RH parasites were monitored after stimulation with 10^-5 M progesterone. (D) GCaMP6f-RH parasites stimulated with estradiol after treatment with BSA-coupled estradiol. (E) DMSO was used as a negative control because all compounds dissolved in DMSO. (F) A23187 (calcium ionophore, 2 μM, Sigma, United States), a mobile ion-carrier, was used as a positive control for the monitoring of intracellular Ca²⁺ concentrations. Each experiment was performed in triplicate with three independent biological replicates.