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. 2018 Jun 12;9:1266. doi: 10.3389/fmicb.2018.01266

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Copyright © 2018 Zhang, Zhang, Fu, Liu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PKG and PI-PLC are important factors in the control of parasite Ca²⁺ signals. (A,B) Calcium concentrations in intracellular parasites were measured after stimulation with progesterone (A) or estradiol (B) in the presence of 100 μM MBP146-8. (C,D) 100 μM U73122 was used to verify that PI-PLC is necessary for the progesterone- (C) or estradiol-induced (D) Ca²⁺ spike. Each experiment was performed in triplicate with three independent biological replicates.