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. 2018 Jun 12;9:1266. doi: 10.3389/fmicb.2018.01266

FIGURE 4.

FIGURE 4

Estrogen modulates microneme secretion and gliding motility. (A) Western blot of parasite supernatants and lysates following treatment with estradiol or progesterone. A23187, positive control; DMSO, negative control; GRA1: control for parasite viability; ESA: excretory-secretory antigens; MIC2 and GRA1 polyclonal antibodies were prepared in our laboratory. (i) Western blot; (ii) statistical results of western blot. (B) Secretion of MIC2 was stimulated by estradiol or progesterone. The MIC2 monoclonal antibody was provided by Silvia N. J. Moreno, University of Georgia. (C) Gliding traces of T. gondii recorded from a representative field of view. Gliding motility is blocked by treatment with progesterone (10-5 mol L) compared with the estradiol (10-8 mol L) and DMSO groups. The SAG1 polyclonal antibody was prepared in our laboratory. (i,ii) progesterone pretreated T. gondii; (iii) estradiol pretreated parasites; (iv) DMSO pretreated parasites; (v) statistical results of gliding traces.