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. 2018 Jun 12;9:628. doi: 10.3389/fphar.2018.00628

FIGURE 7.

FIGURE 7

Total ion chromatogram of Jinshui Huanxian formula (JHF)-containing serum, and the effect of ruscogenin on the differentiation of fibroblast. (A) The 20 compounds were identified in JHF-containing serum: 1, Catalpol; 2, geniposide; 3, leonuride; 4, bilobalide; 5, Peimine; 6, hesperidin; 7, Peiminine; 8, Ginsenoside Re; 9, Ginkgolide B; 10, Ginkgolide A; 11, Anemoside B4; 12, Icraiin; 13, Ophiopogonin D; 14, Ginsenoside Rb1; 15, Ginsenoside Rc; 16, Nobiletin; 17, Methylophiopogonanone B; 18, Paeonol; 19, Ruscogenin; 20, 3,29-Dibenzoyl rarounitriol. (B) The effect of ruscogenin on NIH-3T3 cell viability was detected by MTT analysis. (C,D) The NIH-3T3 cells were treated with TGF-β1 (7.5 ng/ml) and different concentrations of ruscogenin (2.5, 5, 10 μg/ml) for 24 h. Then the protein levels of FN and COL-I in the supernatant of NIH-3T3 cells were detected. Values represented as mean ± SEM. ##P < 0.01, versus control group. ∗∗P < 0.01, ∗P < 0.05, versus model group.