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. 2001 Oct 23;98(22):12374–12378. doi: 10.1073/pnas.221467798

Figure 5.

Figure 5

Properties of 11–36 (1–22) vs. wt λ N (1–22). (A) Binding isotherm for 11–36 (1–22) and wt λ N (1–22) detected by fluorescence of 2AP-2-labeled boxBR in 100 mM KOAc/20 mM Tris⋅OAc. The fluorescence of the RNA is quenched by wt λ N (1–22) and enhanced by 11–36 (1–22). (B) CD spectra of 11–36 (1–22) and wt λ N (1–22). (C) NMR spectra of the BoxBR RNA alone and complex with the wt λ N (1–22) or 11–36 (1–22) peptides. Both peptides protect the G loop nucletide from exchange (10.7 ppm) and give rise to similar amino spectra in the RNA stem.