Figure 2.

NAC alleviated the decrease of MHC levels and the increase of MuRF-1 and MAFbx levels caused by TNF-α treatment. (A) MHC, MuRF-1 and MAFbx expression in C2C12 myotubes were determined by Western blotting; (B) the relative expression of MHC, MuRF-1 and MAFbx in C2C12 myotubes. NC: C2C12 myotubes without any treatment; TNF-α: C2C12 myotubes treated with TNF-α only; TNF-α + NAC: C2C12 myotubes treated with TNF-α and NAC simultaneously. *, P<0.05 versus NC; ^#, P<0.05 versus TNF-α. NAC, N-acetyl-L-cysteine; MHC, myosin heavy chain; MAFbx, muscle atrophy F-box; MuRF1, muscle RING finger-1.