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. 2018 Jun 18;8:9259. doi: 10.1038/s41598-018-27050-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of cilia defects caused by the knockdown of IFT46 and IFT80 in Paramecium. (A) The three coloured lines show different division rates after wild-type Paramecium cells were fed on Day 1, Day 2 and Day 3. The blue line represents the division rate of ND7 RNAi clones as control, the red line represents the division rate of IFT80 RNAi clones, and the green line represents the division rate of IFT46 RNAi clones. (B,C) These two graphs describe the changes in length and number of cilia after wild-type Paramecium cells were treated with different RNAi for 16 or 24 h.