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. 2018 Jun 18;8:9259. doi: 10.1038/s41598-018-27050-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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EM photos show defects of the ciliary structure by the depletion of IFT46 in Paramecium. Photos (A–G) show electron micrographs of sections of cilia in wild-type (WT) cells or in IFT46 RNAi cells after 16 or 24 h of feeding. (A,D) Wild-type. (B,E,F) IFT46 RNAi after 16 h. (C,G) IFT46 RNAi after 24 h. Yellow arrows show short cilia with ill-organised microtubules. Bar: 200 nm.