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. 2018 Jun 18;8:9259. doi: 10.1038/s41598-018-27050-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Whole-genome transcriptomic analysis and RT-qPCR confirmed the efficiency of RNAi methods. The transcription level of IFT46 and IFT80 decreased in the group of RNAi in comparison with control group (empty PPD vector). (A) High throughput RNA seq showed that the expression level of IFT46 is significant decreased in the group of IFT46 RNAi, as well as IFT80 in the group of IFT80 RNAi. (B) Using the expression of tubulin as control, the result of RT-qPCR also showed that the expression of IFT46 and IFT80 are significantly decreased in RNAi group. (C) High throughput RNA seq showed the expression level of different IFT proteins in the group of IFT46 RNAi.