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. 2018 Jun 18;8:9259. doi: 10.1038/s41598-018-27050-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Co-localisation of the IFT46-GFP fusion protein on basal bodies and cilia is displayed by using ID5 and anti-polyglycylated tubulin antibodies, respectively, as markers. (A1,B1) Green fluorescence displays the localisation of the IFT-46 fusion protein. (A2) Basal bodies stained by the ID5 antibody against polyglutamylated tubulin. (B2) Cilia labelled by anti-polyglycylated tubulin antibody. (A3,B3) Merged photos show co-localisation of the IFT46-GFP fusion protein on basal bodies and cilia, and magnifications show details in cilia and basal bodies. Bar: 10 µm.