Fig. 1. MSC-CM promotes osteosarcoma cell resistance to anoikis in vitro and in vivo.
a Saos2 cells were cultured in suspension with 5% bovine serum albumin or DMEM containing 5% FBS or MSC-CM. The number of apoptotic cells was measured by flow cytometry. b Colony formation assay. c Monitoring of osteosarcoma cell survival in vivo using the IVIS 200 system. Data are presented as mean ± standard deviation. *p < 0.05, **p < 0.01, and ***p < 0.001. All in vitro data were obtained from at least three independent experiments. Each group contained 10 animals