Fig. 3. (There is a mistake in Fig.3C, the first group is Saos2-luc,not Sao2-luc,we have uploaded the revised figure 3 in the attachment of this system.)MSC-CM-derived IL-8-induced activation of CXCR1 promotes anoikis resistance in Saos2-lung-M cells.

a, b Anoikis rates of Saos2-luc, Saos2-lung, and Saos2-lung-M cells cultured in suspension. c Comparison of IL-8 secretion by metastatic OS subtypes and parental cells. d Quantification of IL-8 in the MSC-CM. e Anoikis rate of Saos2 cells stimulated with MSC-CM, IL-8, and IL-8 or MSC-CM plus IL-8-neutralizing antibody. f Expression of chemokine receptors on OS cells. g Western blot showing CXCR1 expression in OS cells in suspension and in a normal state. h Quantification of western blot bands. Data are presented as mean ± standard deviation. *p < 0.05, **p < 0.01, and ***p < 0.001. All data were obtained from at least three independent experiments