Fig. 1.

Composition of the PreDicta chip. a Selection process of VP1 peptides representing different RV strains as summarized in Supplementary Table 2. b Multiple sequence alignment of selected VP1 peptides. Left margin: Strain numbers (red circle: RV-A; blue triangle: RV-C, green square: RV-B, black circle: Enterovirus 68). Dots in the alignment represent identical amino acids. Sequence identities (%) with peptide 1 from RV 1 (01p1; top line) are shown for each peptide on the right margin. c Phylogenetic tree of the VP1 peptide sequences in b. d RV microarray layout depicting the positions of recombinant RV proteins/protein fragments, synthetic peptides (VP1-p1, 89VP1-derived peptides), MBP (maltose binding protein), and other control proteins which were spotted in triplicates. Proteins and peptides are designated as described in Supplementary Tables 3–6