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. 2018 Jun 18;8:9311. doi: 10.1038/s41598-018-27633-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative quantification of NcSUT4 and NcNCED3 expression levels using the most and least stable reference genes for normalization in the given experimental conditions. (a) Expression level of NcSUT4 at different developmental stages. Most stable reference genes (SAMDC, PGK) and least stable reference genes (ACT11, EF-1-α) were used, respectively; (b) expression level of NcSUT4 in different tissues. Most stable reference genes (SAMDC, RPL10A) and least stable reference genes (GAPDH, Rubisco) were used, respectively; (c) expression level of NcNCED3 under cold treatment. Most stable reference genes (SAMDC, RPS25) and least stable reference genes (TUA2, EF-1-α) were used, respectively; (d) expression level of NcNCED3 under drought treatment. Most stable reference genes (SAMDC, RPS25) and least stable reference genes (GAPDH, Rubisco) recommended by BestKeeper were used, respectively. The error bars represent the mean of three biological replicates ± SD.